M. Govahi; A. Ghalavand; F. Nadjafi; A. Sorooshzadeh
Abstract
Sage, a popular medicinal plant, is widely used in food and pharmaceutical industries. This research was aimed to study the effects of organic and biological fertilizers on physiological characteristics, yield, and essential oil content of sage (Salvia officinalis L.) under different moisture conditions. ...
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Sage, a popular medicinal plant, is widely used in food and pharmaceutical industries. This research was aimed to study the effects of organic and biological fertilizers on physiological characteristics, yield, and essential oil content of sage (Salvia officinalis L.) under different moisture conditions. The study was conducted in a field experiment in split-plot design with 15 treatments and three replications at Tarbiat Modares University in 2012. Treatments included three irrigation levels (irrigation after depletion of 40% available water, irrigation after depletion of 60% available water, and irrigation after depletion of 80% available water) and five different soil fertility systems (control (no fertilizer) (F0), chemical fertilizer (urea) (Ur), nitrogen fixing bacteria (NFB), vermicompost (V), vermicompost + nitrogen fixing bacteria (V+MFB), allocated to main plots and subplots, respectively. Results indicated that the irrigation regimes had significant effect (p 0.01) on all measured traits. Chlorophyll a, b, and dry matter declined with increasing of water stress, but sugar, proline and essential oil content increased. Results showed that the highest values of all measured traits were related to the vermicompost + nitrogen fixing bacteria treatment. In addition, results showed that the highest essential oil percentage (1.10%) and essential oil yield (20.50 l ha-1) were obtained in vermicompost + nitrogen fixing bacteria treatment under moderate drought stress (I2).
N. Farahani; M. Farzaneh; F. Najafi; H. Rezadoost
Abstract
Secondary metabolites play an important role in plant responses to environmental stresses and some of these compounds are significantly increased under stress conditions. The use of biotic and abiotic elicitors for promoting the production of secondary metabolites reduces the processing time to achieve ...
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Secondary metabolites play an important role in plant responses to environmental stresses and some of these compounds are significantly increased under stress conditions. The use of biotic and abiotic elicitors for promoting the production of secondary metabolites reduces the processing time to achieve high concentrations of the metabolite. In this study, the effects of pathogenic fungus, Rhizoctonia solani (AG2-2) SBUka2, as biotic elicitor, were studied to better understanding of plant reactions to microorganisms and enhance yield and quality of secondary metabolites of Thymus daenensis in tissue culture. In this way, rosmarinic acid and caffeic acid contents of two ecotypes (Ilam and Isfahan) of T. daenensis were determined by High Performance Thin Layer Chromatogharapghy (HPTLC), while essential oil variations were studied by Gas Chromatoghraphy-Mass Spectrometry (GC-MS). The results showed that in the presence of R. solani, rosmarinic acid content of inoculated plant was decreased by 26.82% and 8.25% in Ilam and Isfahan ecotypes, respectively whereas caffeic acid content decreased to 4.51 and 4.16, respectively. The analysis of essential oils exhibited that in the presence of R. solani, the yield of essential oils increased by 33% and 20% in Isfahan and Ilam types, respectively. In addition, the content of some essential oil compositions such as thymol and carvacrol were increased in infected plants whereas other compounds such as para-cymene and gama-terpinene were decreased.
